A total of 65 players are taking part in the 2016 Johor Open. Titled players comprise 8 GMs, 11 IMs and 8 FMs. After the first round, all the top seeds won as expected.

The only notable "upset" is Daler Vakhidov (1983) defeating IM Sean WInshand Kushendi (2408).

Top seed in this tournament is 21 year Iranian GM Idani Pouya.

Bo.	No.		Name	Rtg	Result		Name	Rtg	No.
1	35		Lye Lik Zang	2067	0 - 1	GM	Idani Pouya	2534	1
2	2	GM	Pourramezanali Amirreza	2525	1 - 0	FM	Ismail Ahmad	2057	36
3	4	IM	Kuybokarov Temur	2457	1 - 0		Pham Minh Hieu	2026	37
4	38	FM	Wiwatanadate Poompong	2024	0 - 1	GM	Fominyh Alexander	2437	5
5	6	GM	Vahidov Tair	2432	½ - ½		Abramowski Joerg	2017	39
6	40		Khandhar Kaushal	2006	0 - 1	IM	Nolte Rolando	2419	7
7	42		Daniel Hermawan Lumban Tobing	1993	0 - 1	IM	Nguyen Van Huy	2416	8
8	43		Vakhidov Daler	1983	1 - 0	IM	Cuhendi Sean Winshand	2408	10
9	11	GM	Schebler Gerhard	2406	1 - 0	CM	Tan Jun Ying	1978	44
10	45		Nguyen Truong Bao Tran	1954	0 - 1	GM	Cao Sang	2402	12
11	13	GM	Laxman R.R.	2389	1 - 0	WCM	Isha Sharma	1935	46
12	47		Tan Kok Liang	1901	0 - 1	IM	Lioe Dede	2372	14
13	49		Tan Soon Wei Dawson	1889	0 - 1	FM	Dang Hoang Son	2350	15
14	16	IM	Soozankar A.M.	2345	1 - 0		Kebbedies Frank	1877	50
15	51		Singh Kabir	1829	0 - 1	FM	Pitra Andika	2308	17
16	53		Pereira Johnharry	1721	0 - 1	IM	Ramnath Bhuvanesh.R	2297	18
17	19	IM	Sharma Dinesh K.	2288	1 - 0		Cheah Kah Hoe	1665	54
18	55		Mohd Radzuan Muhd Harith Farh	1626	0 - 1	FM	Lim Zhuo Ren	2276	20
19	22	FM	Lee Qing Aun	2249	1 - 0		Ang Jing Xuan	1599	56
20	57		Tarimo Didik Widiarso	1571	½ - ½		Norbaev Nabijon	2246	23
21	24	IM	Chan Peng Kong	2225	1 - 0		Sim Jia Ru	1569	58
22	59	WCM	Chua Jia-Tien	1565	½ - ½	WIM	Tejaswini Sagar	2204	25
23	26	FM	Low Zhen Yu Cyrus	2200	1 - 0		Lim Lui Lung	1557	60
24	62		Ng Wee Lok Bryan	1532	0 - 1		Navalgund Niranjan	2153	27
25	63		Lim Zen Hong	1306	½ - ½	WFM	Citra Dewi Ardhiani Anastasia	2133	29
26	30		Yeap Eng Chiam	2124	1 - 0		Jaffar Wazir	0	64
27	32		Molofej Denis	2089	1 - 0		Djumaev Bakhtiyor	1999	41
28	34		Wika Hardiyanto	2071	0 - 1	GM	Neelotpal Das	2463	3
29	9	IM	Vo Thanh Ninh	2413	1 - 0	FM	Pratama Constantius Leonardo	2071	33
30	31	CM	Abdullah Mohd Kamal	2117	1 - 0		Nithyalakshmi Sivanesan	1892	48
31	28	FM	Wong Yinn Long	2138	1 - 0		Narayanan Renitha	1780	52
32	21	FM	Alfred Nathan S W	2275	1 - 0		Doshi Devansh Sanjay	1535	61
33	65		Wee Han Xia	0	1		bye